Detailed morphological and kinetic studies of cellulose biosynthesis from Leifsonia soli
Bacterial cellulose is a natural biopolymer that finds wide application in areas such as biomedical, food and food packaging, paper industries, cosmetic industries, etc. due to its high purity. A novel type of study has been done which is based on the concepts of kinetics and morphology of produced cellulose by the Leifsonia soli. With the help of atomic force microscopy and field emission scanning electron microscopy, it has been observed that the cellulose layer formation in the culture flask was increasing up to the 7th day of incubation. Further, the semi-crystalline nature of cellulose has been found through an x-ray diffraction technique and it revealed that the structure of cellulose was allomorph of I? and I?. Additionally, the growth kinetics of bacteria was also co-related to cellulose production along with that the actual mechanism of cellulose formation by bacteria within the culture medium during fermentation and its prediction on maximum cellulose synthesis in a non-invasive manner has been predicted in this study through different microscopic evaluation.
Publication date: 01/03/2022
Author: Akanksha Rastogi, Sumita Sahoo, Tapas Kumar Bandyopadhyay, Rabibrata Mukherjee, Rintu Banerjee